METHOXYRESORUFIN
Synonym(s):7-Methoxy-3H-phenoxazin-3-one;Methoxyresorufin;O7-Methylresorufin
- CAS NO.：5725-89-3
- Empirical Formula: C13H9NO3
- Molecular Weight: 227.22
- MDL number: MFCD00037663
- SAFETY DATA SHEET (SDS)
- Update Date: 2026-04-24 14:58:06
What is METHOXYRESORUFIN?
Description
Methoxyresorufin is a fluorogenic substrate for the cytochrome P450 (CYP) isoforms CYP1A1 and CYP1A2. Methyoxyresorufin is O-demethylated by CYP1A1/2, releasing resorufin, which displays excitation/emission maxima of 570/580 nm, respectively. The appearance of resorufin fluorescence can be used to quantify CYP1A1/2 activity.
The Uses of METHOXYRESORUFIN
Substrate for Cytochrome P-450. METHOXYRESORUFIN is used for various investigations including the differentiatiation of cytochrome P-450 isozymes.
The Uses of METHOXYRESORUFIN
Resorufin methyl ether have been used as substrate in the incubation mixture, during the determination of cytochrome P4501A activities such as ethoxyresorufin O-deethylase (EROD) and methoxyresorufin O-demethylase(MROD) in liver microsomes, using high performance liquid chromatography(HPLC).
The Uses of METHOXYRESORUFIN
Used to differentiate isozymes of cytochrome P-450
What are the applications of Application
Resorufin methyl ether is a fluorometric substrate
Biochem/physiol Actions
Fluorimetric substrate for cytochrome P450 linked enzymes.
Properties of METHOXYRESORUFIN
| Melting point: | ≥220 °C(lit.) |
| Boiling point: | 378.8±42.0 °C(Predicted) |
| Density | 1.33±0.1 g/cm3(Predicted) |
| storage temp. | 2-8°C |
| solubility | DMF: soluble |
| pka | 1.42±0.20(Predicted) |
| form | Powder |
| color | Brown to reddish brown |
| BRN | 209529 |
Safety information for METHOXYRESORUFIN
Computed Descriptors for METHOXYRESORUFIN
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