KiCqStart SYBR Green qPCR ReadyMix

The Uses of KiCqStart SYBR Green qPCR ReadyMix

Instrument Compatibility:
Different real-time PCR systems employ different strategies for normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument. KiCqStart SYBR Green qPCR ReadyMix does not contain an internal reference dye. Compatible instruments include:

• Bio-Rad^? CFX384^?
• Bio-Rad CFX96^?
• Bio-Rad MiniOpticon^?
• Bio-Rad/MJ Chromo4^?
• Bio-Rad/MJ Opticon 2
• Bio-Rad/MJ Opticon^?
• Cepheid SmartCycler^?
• Eppendorf Mastercycler^? ep realplex
• Eppendorf Mastercycler ep realplex2 s
• Illumina Eco qPCR
• Qiagen/Corbett Rotor-Gene^? 3000
• Qiagen/Corbett Rotor-Gene 6000
• Qiagen/Corbett Rotor-Gene Q
• Roche LightCycler^? 480

The Uses of KiCqStart SYBR Green qPCR ReadyMix

KiCqStart^? SYBR^? Green qPCR ReadyMix^? has been used for the amplification and quantification of DNA in real-time PCR (qPCR) assay. It has also been used in the amplification and quantification of transcripts in 2-step quantitative reverse transcription polymerase chain reaction (qRT-PCR).

General Description

KiCqStart SYBR Green qPCR ReadyMix is a 2X concentrated, ready-to-use reaction cocktail that contains all components, except primers and template, for real-time quantitative PCR (qPCR) This unique combination of proprietary buffer, stabilizers, and Hot-Start Taq DNA polymerase delivers maximum PCR efficiency, sensitivity, specificity and robust fluorescent signal using fast, or conventional, cycling protocols with SYBR Green qPCR.

Highly specific amplification is crucial to successful qPCR with SYBR Green I dye technology because this dye binds to and detects any dsDNA generated during amplification. Hot-Start Taq DNA polymerase is antibody mediated to be inactive prior to the initial PCR denaturation step. The optimized formulation includes SYBR Green I dye, an antibody-mediated Hot-Start Taq DNA polymerase, dNPTs, MgCl₂ and proprietary buffers and stabilizers.