KAPA3G Plant PCR Kit

The Uses of KAPA3G Plant PCR Kit

KAPA3G Plant PCR Kit has been used for:

• Amplification of fragments up to 5 kb in size from purified plant DNA, extracted with commercial kits or cetyl trimethyl ammonium bromide (CTAB)-based methods
• Direct polymerase chain reaction (PCR) from leaf discs, seed samples, and other plant tissue types
• PCR from crude plant DNA extracts, prepared from leaf and/or seed material
• amplification of 16SrRNA
• an ultra-rapid real-time reverse transcriptase (RT)-PCR assay

General Description

KAPA3G Plant PCR Kits are designed for PCR of plant-derived DNA, using either purified DNA or DNA prepared by crude extraction methods (crude sample PCR). In addition, the KAPA3G Plant PCR Kit can be used to amplify DNA from plant material added directly to the PCR (direct PCR). The KAPA3G Plant PCR Kit contains KAPA3G DNA Polymerase, a novel, third-generation (3G) enzyme which was engineered via a process of directed evolution for improved tolerance to common plant-derived PCR inhibitors such as polyphenolics and polysaccharides.The KAPA3G Plant PCR Kits are optimized for fast and efficient amplification of plant DNA from crude samples, DNA-containing carry-over inhibitors from crude extraction methods, and purified DNA.
KAPA3G Plant DNA Polymerase is a blend of the engineered A-family KAPA3G DNA Polymerase and a modified B-family DNA Polymerase. The enzyme blend is combined with proprietary antibodies to inactivate the enzymes prior to the first denaturation step. The fidelity of the KAPA3G Plant DNA Polymerase is 4-10 times higher than that of wild-type Taq.