KAPA SYBRFAST One-Step

The Uses of KAPA SYBRFAST One-Step

KAPA SYBR^? FAST One-Step

• Gene expression analysis
• Low-copy gene detection
• Microarray validationmiRNA research
• Gene knockdown validation
• Real-time quantitative reverse transcription-PCR (qRT-PCR)
• RNAi and miRNA research

The Uses of KAPA SYBRFAST One-Step

KAPA SYBR^? FAST One-Step is suitable for:

• One-step quantitative reverse transcriptase PCR (qRT-PCR)
• Gene expression analysis
• Low-copy gene detection
• Microarray validation
• miRNA research
• Gene knockdown validation

General Description

KAPA SYBR FAST One-Step qRT-PCR Kits are designed for optimal performance in stringent qRT-PCR reaction conditions, exhibiting dramatic improvements in sensitivity, specificity, reaction efficiency, and fluorescence. The kit is optimized for rapid one-step, one-tube RNA quantification, reducing experimental variation and contamination with a convenient qRT-PCR protocol.

KAPA SYBR FAST One-Step qRT-PCR Kits contain M-MuLV reverse transcriptase, RNase inhibitor, and a novel DNA polymerase evolved via directed evolution.

Biochem/physiol Actions

KAPA SYBR? FAST DNA Polymerase displays no enzymatic activity at ambient temperature. This prevents the formation of misprimed products and primer-dimers during reaction setup prior to the first denaturation step and results in high PCR specificity and accurate quantification. Moloney murine leukemia virus reverse transcriptase (M-MuLV-RT) has a high affinity for RNA and is optimized for cDNA synthesis at 42°C. The RNase Inhibitor safeguards against degradation of RNA target due to RNase contamination.