HOECHST 34580

Synonym(s):N,N-Dimethyl-4-[5-(4-methyl-1-piperazinyl)[2,5′-bi-1H-benzimidazol]-2′-yl]benzenamine trihydrochloride;HOE 34580

CAS NO.：23555-00-2
Empirical Formula: C27H29N7
Molecular Weight: 451.57
MDL number: MFCD25541584
SAFETY DATA SHEET (SDS)
Update Date: 2026-04-21 10:31:43

What is HOECHST 34580?

Biological Activity

Hoechst 34580 (HOE 34580) is a cell-permeable blue fluorescent dye used to stain DNA and cell nuclei. It is also a good candidate for the treatment of Alzheimer's disease by inhibiting the formation of amyloid beta (Aβ) with an IC50 value of 0.86 μM.

in vitro

Hoechst 34580 prefers to locate near hydrophobic regions with binding affinity with the inhibition constant IC ₅₀ of 0.86±0.05 μM for Hoechst 34580. It is a good candidate for treating the Alzheimer's disease by inhibiting Aβ formation. 50 μM Aβ42 solutions co-incubated with 100, 25, 12.5, 3.125, 0.78, and 0.1, 0.01 μM Hoechst 34580 at 37 °C for 70 h. Hoechst 34580 can inhibit the aggregation of Aβ42 in a dose- dependent manner. And the half-maximal concentration (IC ₅₀ ) is examined. The IC ₅₀ is obtained by measuring the concentration of Hoechst 34580 while maintaining the Aβ42 concentration which gave 0.86 ±0.05 μM for Hoechst 34580.

Properties of HOECHST 34580

Melting point:	>300℃
Boiling point:	733.2±70.0 °C(Predicted)
Density	1.280±0.06 g/cm3(Predicted)
storage temp.	2-8°C(protect from light)
solubility	Soluble in dimethyl sulfoxide, water
form	powder
pka	10.97±0.10, most acidic, temperature: 25 °C; 7.66 ± 0.42, most basic(at 25℃)
color	Yellow to green
λmax	392nm
Biological Applications	Detecting malaria infected red blood cells,polynucleotides; nucleic acid amplification; nucleic acid sequencing

Safety information for HOECHST 34580

Computed Descriptors for HOECHST 34580

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