High Pure miRNA Isolation Kit

Molecular Weight: 0
Update Date: 2025-01-27 09:38:02

What is High Pure miRNA Isolation Kit?

The Uses of High Pure miRNA Isolation Kit

The High Pure miRNA Isolation kit rapidly purifies RNA that is suitable for direct use in many downstream applications:

Northern blotting
cDNA synthesis
Primer extension
miRNA array hybridization
Relative quantification of miRNA with RT-PCR using, for example, the LightCycler^? 480 System

General Description

In the presence of a chaotropic salt, RNA binds selectively to special glass fibers prepacked in the High Pure Spin Filter Tube. Bound RNA is purified in a series of rapid wash-and-spin steps to remove contaminating salts, proteins, and other cellular impurities, and then eluted using a low-salt solution. By lowering the concentration of Binding Enhancer during the binding step in the two-column protocol, the small RNA-containing miRNA passes the first column unbound. When the concentration of Binding Enhancer is increased, the small RNA fraction can be bound to a second High Pure Spin Filter Tube.

Safety information for High Pure miRNA Isolation Kit

Signal word	Danger
Pictogram(s)	Corrosion Corrosives GHS05 Exclamation Mark Irritant GHS07 Health Hazard GHS08
GHS Hazard Statements	H314:Skin corrosion/irritation H317:Sensitisation, Skin H334:Sensitisation, respiratory H335:Specific target organ toxicity, single exposure;Respiratory tract irritation H412:Hazardous to the aquatic environment, long-term hazard
Precautionary Statement Codes	P201:Obtain special instructions before use. P260:Do not breathe dust/fume/gas/mist/vapours/spray. P280:Wear protective gloves/protective clothing/eye protection/face protection. P303+P361+P353:IF ON SKIN (or hair): Remove/Take off Immediately all contaminated clothing. Rinse SKIN with water/shower. P308+P313:IF exposed or concerned: Get medical advice/attention.

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