DAF-FM DA
Synonym(s):3-Amino, 4-aminomethyl-2ʹ,7ʹ-difluorofluorescein Diacetate;4-Amino-5-(N-methylamino)-3′,6′-bis(acetyloxy)-2′,7′-difluoro-spiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3-one;4-Amino-5-methylamino- 2′,7′-difluorofluorescein diacetate;DAF-FM DA - CAS 254109-22-3 - Calbiochem;Diaminofluorescein-FM diacetate
- CAS NO.：254109-22-3
- Empirical Formula: C25H18F2N2O7
- Molecular Weight: 496.42
- MDL number: MFCD16872050
- SAFETY DATA SHEET (SDS)
- Update Date: 2026-05-11 15:21:01
What is DAF-FM DA?
The Uses of DAF-FM DA
DAF-FM DA has been used to measure the nitric oxide (NO) levels in liver tissue homogenates. It has also been used in NO staining for detection of NO from human umbilical vein endothelial cells (HUVECs).
The Uses of DAF-FM DA
DAF-FM diacetate is an important reagent for quantification of low concentrations of nitric oxide in solution. This compound is essentially nonfluorescent until it reacts with NO to form a fluorescent benzotriazole. With excitation/emission maxima of 495
What are the applications of Application
DAF-FM DA (cell permeable) is a cell-permeable analog of DAF-FM that acts as a cell permeable NO indicator
Biochem/physiol Actions
DAF-FM DA is a cell-permeable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM and DAF-FM DA are more sensitive to nitric oxide (NO) compared with DAF-2 and more photo-stable and less pH sensitive. DAF-FM DA permeates well into living cells and is rapidly transformed into water-soluble DAF-FM by cytosolic esterases.
Storage
Store at -20°C
Properties of DAF-FM DA
| Boiling point: | 695.7±55.0 °C(Predicted) |
| Density | 1.56±0.1 g/cm3(Predicted) |
| storage temp. | -20°C |
| solubility | Soluble in dimethyl sulfoxide |
| form | Pale yellow solution |
| pka | 4.20 ± 0.20, most basic, temperature:
25 °C |
| color | clear clear, colorless |
| λmax | 365 nm (MeOH) |
| Biological Applications | Detecting bacteria/microorganisms; nitric oxide indicator, |
Safety information for DAF-FM DA
Computed Descriptors for DAF-FM DA
New Products
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