Azidoacetic acid NHS ester
Synonym(s):(2,5-Dioxopyrrolidin-1-yl) 2-azidoacetate;1-[(Azidoacetyl)oxy]pyrrolidine-2,5-dione
- CAS NO.：824426-32-6
- Empirical Formula: C6H6N4O4
- Molecular Weight: 198.14
- MDL number: MFCD24452499
- SAFETY DATA SHEET (SDS)
- Update Date: 2026-01-13 11:24:42
What is Azidoacetic acid NHS ester?
Description
Azidoacetic acid NHS ester is a compound containing an azide group and an NHS ester. The azide group can react with alkyne, BCN, DBCO via Click Chemistry to yield a stable triazole linkage. The NHS ester can be used to label the primary amines (-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules.
The Uses of Azidoacetic acid NHS ester
Azidoacetic acid NHS ester is a simple azido-containing reagent with an N-hydroxysuccinimide (NHS) ester that can be reacted with primary amines at pH 7-9 forming a stable amide bond. The azide allows for chemoselective ligation with an alkyne or cyclooctyne. It is generally used to functionalize amines and amino acids with azidoacetyl groups. It is generally used in click chemistry applications.
Properties of Azidoacetic acid NHS ester
| Melting point: | 132-133o C |
| storage temp. | -20°C |
| solubility | Chloroform (Slightly) |
| form | powder or crystals |
| color | White to Off-White |
Safety information for Azidoacetic acid NHS ester
| Signal word | Danger |
| Pictogram(s) |
 Flame Flammables GHS02 |
| GHS Hazard Statements |
H242:Self-reactive substances and mixtures; and Organic peroxides |
| Precautionary Statement Codes |
P210:Keep away from heat/sparks/open flames/hot surfaces. — No smoking. P234:Keep only in original container. P235:Keep cool. P240:Ground/bond container and receiving equipment. P370+P378:In case of fire: Use … for extinction. P403:Store in a well-ventilated place. |
Computed Descriptors for Azidoacetic acid NHS ester
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