ANTI-HUMAN VIMENTIN

The Uses of ANTI-HUMAN VIMENTIN

Monoclonal Anti-Vimentin antibody has been used in western blotting and immunocytochemistry.

The Uses of ANTI-HUMAN VIMENTIN

Monoclonal Anti-Vimentin antibody can be used in immunocytochemistry and immunohistochemical staining. It can also be used in immunofluorescence staining (diluted 1: 500) .

The Uses of ANTI-HUMAN VIMENTIN

Monoclonal anti-vimentin antibody can be used in the following:

• Immunocytochemical localization of vimentin in normal and pathological tissue of mesenchymal origin.
• It can also be used in immunoblotting
• Immunofluorescence
• Further, anti-vimentin antibody can be used to distinguish tumors and metastatic lesions derived from sarcomas, lymphomas, and melanomas.

General Description

Vimentin is encoded by a single-copy gene mapped to human chromosome 10p13. 53kDa vimentin protein belongs to type III intermediate filament (IF) family and is specifically expressed in normal mesenchymal cells. Vimentin protein with 466 amino acids is characterized with a highly conserved a-helical “rod” domain that is flanked by non-α-helical amino- terminal “head” and carboxy-terminal “tail” domain.

Biochem/physiol Actions

Vimentin is the major cytoskeletal component in immature glia. It is an intermediate filament protein and serves as a substrate for several caspases. The cleavage of this protein by caspase dismantles intermediate filaments and promotes apoptosis.