ANTI-C-ERBB-4 ANTIBODY, MOUSE MONOCLONAL

The Uses of ANTI-C-ERBB-4 ANTIBODY, MOUSE MONOCLONAL

Anti-c-erbB-4 antibody, Mouse monoclonal has been used in immunoprecipitation. It has also been used in studies on receptor turnover, phosphorylation, ligand binding and induction of differentiation of cultured breast cancer cells.

General Description

Monoclonal Anti-c-erbB-4 (erb-b2 receptor tyrosine kinase 4) (HER-4) (mouse IgG1 isotype) is derived from the HER4-36 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a purified extracellular domain of human erbB-4 from transfected cells. The human ErbB-4 oncogene (also known as HER4) is a 180–185 kDa receptor that binds to a family of isoforms, collectively known as neuregulins. This gene belongs to epidermal growth factor receptor family and is expressed mainly in several breast carcinoma cell lines, heart, brain, cerebellum, pituitary and in normal skeletal muscle.

Biochem/physiol Actions

The epidermal growth factor receptor (ErbB/HER) family of transmembrane receptor tyrosine kinases significantly participates in defining cell lineage during mesenchyme-epithelial inductive processes and in the neuro-muscular and glia-Schwann cells interactions. It plays a crucial role as a cell surface receptor for neuregulins. It can also bind with erbB -2 to forms heterodimers and can modulate ligand affinity. The catalytic domain is important for the intrinsic tyrosine kinase activity and shows greater degree of homology. Certain ligands and receptors of the family contribute to a relatively virulent phenotype of some human tumors; most notable are carcinomas of secretory epithelia. ErbB-4 also binds to related group of molecules, termed neuregulin 2 (NRG2)(which also binds to erbB-3) and NRG3 (which binds exclusively to erbB-4). Two other ligands, betacellulin and the heparin-binding EGF-like growth factor, bind to both erbB-1 and erbB-4. ErbB-4 forms heterodimers with erbB-2, resulting in the modulation of its ligand affinity.