Anti-AKR1C3 antibody, Mouse monoclonal

The Uses of Anti-AKR1C3 antibody, Mouse monoclonal

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

General Description

Anti-AKR1C3 antibody, Mouse monoclonal (humanα - hydroxysteroid dehydrogenase, type 2,3; 17β- hydroxysteroid dehydrogenase, type 5) (mouse IgG1 isotype) is derived from the hybridoma NP6.G6. A6 produced by the fusion of mouse myeloma cells (SP20 cells) and splenocytes from BALB/c mice immunized with human AKR1C3 protein. Oxidoreduction of natural and foreign substrates is performed by three enzymes superfamilies, one of them being the AKRs (aldo-keto reductases) family. This family contains 114 proteins that are expressed in prokaryotes and eukaryotes and is divided into 14 subfamilies (AKR1-AKR14). The AKR1 family is the largest among the different fourteen families and contains the aldose reductases, aldehyde reductases, hydroxysteroid dehydrogenases, and steroid 5 β-reductases. AKR1Cs are expressed in many tissues.

Biochem/physiol Actions

In humans AKR1C3 (aldo-keto reductase family 1 member C3) including AKR1C1,2 and 4, catalyze the reduction of the androgen 5α-dihydrotestosterone (DHT) into inactive 3β or 3α-androstane diol (3α/β-Diol). AKR1C1-4 isoforms also display 3α[17β]-hydroxysteroid oxidase activity using 3α-Diol as a substrate, in vitro. In non-small cell lung carcinoma, AKR1Cs expression is dramatically increased. Due to their product profile and discrete tissue localization, they may regulate the level of active androgens, estrogens, and progestins in target tissues.