Acodazole

Originator

Acodazole Hydrochloride ,ZYF Pharm

The Uses of Acodazole

Antineoplastic.

Manufacturing Process

An 82.0 g (0.5 mole) of 5-nitrobenzimidazole in 900 ml of ethanol was reduced over 4.0 g of 5% Pd/C catalyst containing 50% H2O. After filtration of the catalyst, 65.0 g (0.5 mole) of ethyl acetoacetate, 20.0 g of anhydrous calcium sulfate, and 0.5 ml of HOAc was added. After filtration, the solution was concentrated in vacuo till a solid remained. The product was filtered and washed with fresh ethanol and air-dried. The yield of ethyl 3-(5- benzimidazolylamino)crotonate was 84.0 g (69%), melting point 160°-162°C.
40.0 g of ethyl 3-(5-benzimidazolylamino)crotonate was added to 80 ml of boiling Dowtherm? and the boiling was continued for 5 min. The product separated upon cooling. The product was filtered, washed with Dowtherm? and then acetone and air-dried. The yield of 7-methyl-9-imidazo[4,5- f]quinolinol was 29.0 g (91%), melting point 345°-347°C.
Into a 22 L, 4-necked flask set in a tub and equipped with a stirrer, an air condenser (drying tube), thermometer, and dropping funnel was placed POCl3 (4590 ml). The 7-methyl-9-imidazo[4,5-f]quinolinol (1062.0 g, 5.33 moles) was added with no heating effect noted. Dimethylformamide (4690 ml) was added dropwise over a 2.5 h period at a rate to control the temperature below 85°C. The resulting viscous solution was allowed to stand overnight at room temperature and then added cautiously to ice to a total volume of ca. 50 L. The resulting solution was then adjusted to a pH of 7 to 8 by the addition of NaOH pellets (9771.0 g). More ice was added as needed to keep the temperature below 45°C. The resulting precipitate was collected by filtration, washed well by stirring in water (3x20 L) and dried at 60°C to yield 1107.0 g (95.5%) of 9-chloro-7-methylimidazo[4,5-f]quinolone. To 500 ml of acetic anhydride was added portionwise, 100.0 g (0.658) of Nmethyl- p-nitroaniline. Following the addition, the solution was heated on a steam bath for 2 h, then stirred overnight at room temperature. The white precipitate of the N-methyl-4-nitroacetanilide was collected by filtration, washed with ether and air-dried to give 53.0 g, melting point 153°-156°C. The filtrate was concentrated in vacuum to give another 61.0 g, melting point 150°-154°C.
A mixture of 114.0 g (0.587 m) of N-methyl-4-nitroacetanilide and 800 ml of ethanol was shaken with hydrogen over one teaspoon of Raney active nickel catalyst in water. A pressure drop of 127 psi was recorded (calc. 118 psi). The catalyst was removed by filtration and the ethanol filtrate refluxed overnight with 127.0 g (0.587 m) of the 9-chloro-7-methylimidazo[4,5-f]quinolone. The mixture was chilled, filtered, washed with ether and air-dried to give 75.5 g of 9-[p-(N-methylacetamido)anilino]-7-methyl-1-H-imidazo[4,5-f]quinoline hydrochloride sesquihydrate, melting point 315°-318°C (recrystallized from 4,000 ml of MeOH). By treatment of 9-p-(N-methylacetamido)anilino-7-methyl-1H-imidazo[4,5- f]quinolone hydrochloride sesquihydrate with NaOH may be produced the 9-p- (N-methylacetamido)anilino-7-methyl-1H-imidazo[4,5-f]quinolone.

Therapeutic Function

Antineoplastic