A549 Cells GFP-EGFR

The Uses of A549 Cells GFP-EGFR

A549 Cells GFP-EGFR is an A549 cell line in which the genomic EGFR gene has been endogenously tagged with a Green Fluorescent Protein gene (GFP) using CompoZr^? Zinc Finger Nuclease technology. Integration resulted in endogenous expression of the fusion protein in which GFP is attached to the C-terminus of EGFR. Fluorescence imaging shows characteristic EGFR membrane expression. Upon the addition of EGF, the cell line shows redistribution of EGFR from the cell membrane to endosomes, making it useful for high content screening of compounds. For example, the redistribution can be abolished by a selective inhibitor of EGFR, Tyrphostin AG 1478. This stable cell line was expanded from a single clone. The target′s gene regulation and corresponding protein function are preserved in contrast to cell lines with overexpression via an exogenous promoter.

General Description

Glucocorticoid is necessary for A549 cell induction to respond to OSM (oncostatin M), interleukin (IL-6) and fibroblast conditioned medium. The EGFR gene is mapped to human chromosome 7p11.2 and encodes a 170?kDa receptor protein belonging to the HER family of receptor tyrosine kinases. Egfr is localized to epithelial cell surface.