4-Hydroxybenzhydrazide

Synonym(s):p-Hydroxybenzoic acid hydrazide;4-Hydroxybenzhydrazide;4-Hydroxybenzoic hydrazide;4-Hydroxybenzoylhydrazine

CAS NO.：5351-23-5
Empirical Formula: C7H8N2O2
Molecular Weight: 152.15
MDL number: MFCD00007605
EINECS: 226-326-5
SAFETY DATA SHEET (SDS)
Update Date: 2025-01-27 09:38:02

What is 4-Hydroxybenzhydrazide?

Chemical properties

white to slightly beige fine crystalline powder

The Uses of 4-Hydroxybenzhydrazide

4-Hydroxybenzhydrazide is a reagent for the rapid automated determination of serum glucose.It can be used as an indicator for the determination of carbohydrates. The acid-hydrolyzable side chains of tissue (liver) bound 4-Hydroxybenzhydrazide can be used as markers to monitor its residues in chickens.

The Uses of 4-Hydroxybenzhydrazide

4-Hydroxybenzhydrazide has been used:
for determining the concentration of maltose from starch digestion
for analysis of sugars from fructan containing food hydrolysate
in colorimetric assay for reducing sugar estimation from processed feedstuff

Biochem/physiol Actions

4-Hydroxybenzhydrazide (PAHBAH), is used for assaying α-amylase and glucoamylase activities. The assay based on PAHBAH measures non-specifically the free reducing sugar groups present in samples at 415nm colorimetrically.

Properties of 4-Hydroxybenzhydrazide

Melting point:	264-266 °C (dec.)(lit.)
Boiling point:	274.61°C (rough estimate)
Density	1.2804 (rough estimate)
refractive index	1.5200 (estimate)
storage temp.	Store at +15°C to +25°C.
solubility	26.5g/l
pka	8.57±0.15(Predicted)
form	Fine Crystalline Powder
color	White to slightly beige
λmax	245nm(MeOH)(lit.)
BRN	473052
CAS DataBase Reference	5351-23-5(CAS DataBase Reference)
NIST Chemistry Reference	P-hydroxybenzoic acid, hydrazide(5351-23-5)

Safety information for 4-Hydroxybenzhydrazide

Signal word	Warning
Pictogram(s)	Exclamation Mark Irritant GHS07
GHS Hazard Statements	H315:Skin corrosion/irritation H319:Serious eye damage/eye irritation H335:Specific target organ toxicity, single exposure;Respiratory tract irritation
Precautionary Statement Codes	P261:Avoid breathing dust/fume/gas/mist/vapours/spray. P264:Wash hands thoroughly after handling. P264:Wash skin thouroughly after handling. P271:Use only outdoors or in a well-ventilated area. P280:Wear protective gloves/protective clothing/eye protection/face protection. P302+P352:IF ON SKIN: wash with plenty of soap and water. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing.