1-Methoxy-5-methylphenazinium methyl sulfate

Synonym(s):1-Methoxyphenazine methosulfate

CAS NO.：65162-13-2
Empirical Formula: C15H16N2O5S
Molecular Weight: 336.36
MDL number: MFCD00040648
EINECS: 265-579-6
SAFETY DATA SHEET (SDS)
Update Date: 2025-12-11 08:41:34

1-Methoxy-5-methylphenazinium methyl sulfate Structural

What is 1-Methoxy-5-methylphenazinium methyl sulfate?

The Uses of 1-Methoxy-5-methylphenazinium methyl sulfate

1-Methoxy-5-methylphenazinium methyl sulfate (PMS) has a redox potential of +63 mV. PMS is often used as an electron carrier for NADH tetrazolium and is a useful reagent for NAD(P)H-tetrazolium-based analytical systems. It is mainly used in the cytotoxic proliferation reagent CCK-8 experiment.

What are the applications of Application

1-Methoxy-5-methylphenazinium methyl sulfate is a photochemically stable mediator of electron transport that works on various electron acceptors including tetrazolium dyes.

Mechanism of action

1-Methoxy-5-methylphenazinium methyl sulfate contains WST-8 [chemical name: 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfonic acid benzene)-2H-tetrazolyl monosodium salt], which is reduced to a highly water-soluble yellow formazan dye by dehydrogenase in cells under the action of the electron carrier 1-methoxy-5-methylphenazinium dimethyl sulfate. The amount of formazan generated is proportional to the number of living cells. Therefore, this property can directly analyze cell proliferation and toxicity. The main uses of this reagent are drug screening, cell proliferation assay, cytotoxicity assay, and tumour drug sensitivity test.

Properties of 1-Methoxy-5-methylphenazinium methyl sulfate

Melting point:	172 °C
storage temp.	Inert atmosphere,Room Temperature
solubility	Water: 1 mg/ml
form	powder to crystal
color	Orange to Brown to Dark purple
InChI	InChI=1S/C14H13N2O.CH4O4S/c1-16-11-7-4-3-6-10(11)15-14-12(16)8-5-9-13(14)17-2;1-5-6(2,3)4/h3-9H,1-2H3;1H3,(H,2,3,4)/q+1;/p-1
CAS DataBase Reference	65162-13-2(CAS DataBase Reference)

Safety information for 1-Methoxy-5-methylphenazinium methyl sulfate

Signal word	Warning
Pictogram(s)	Exclamation Mark Irritant GHS07 Health Hazard GHS08
GHS Hazard Statements	H302:Acute toxicity,oral H315:Skin corrosion/irritation H319:Serious eye damage/eye irritation H341:Germ cell mutagenicity H351:Carcinogenicity H373:Specific target organ toxicity, repeated exposure
Precautionary Statement Codes	P202:Do not handle until all safety precautions have been read and understood. P260:Do not breathe dust/fume/gas/mist/vapours/spray. P301+P312:IF SWALLOWED: call a POISON CENTER or doctor/physician IF you feel unwell. P302+P352:IF ON SKIN: wash with plenty of soap and water. P305+P351+P338:IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continuerinsing. P308+P313:IF exposed or concerned: Get medical advice/attention.